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creator smart cdna library construction kit  (TaKaRa)


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    TaKaRa creator smart cdna library construction kit
    Creator Smart Cdna Library Construction Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 3003 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/creator+smart+cdna+library+construction+kit/SMART+cDNA+Library+Construction+Kit/pm41957118-38-11-17
    Average 96 stars, based on 3003 article reviews
    creator smart cdna library construction kit - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Construct:

    Article Title: Bioinformatic analysis of ESTs collected by Sanger and pyrosequencing methods for a keystone forest tree species: oak
    Article Snippet: For each library devoted to Sanger sequencing, total RNA was extracted following the procedure described by Le Provost et al. [ ] with a final purification step using the RNAeasy kit (QIAGEN, Courtaboeuf, France). .. Depending on the quantity of each tissue, cDNA libraries were constructed using either CloneMiner cDNA Library Construction Kit (Invitrogen Corporation, Carlsbad, CA, USA) and Stratagene cDNA synthesis kit (Stratagene, La Jolla, CA, USA) when plant material was abundant, or Creator SMART cDNA Library Construction Kit (Clontech Laboratories Inc., Mountain View, CA, USA) when plant material was limiting. .. For libraries constructed with CloneMiner kit, mRNA was isolated from 200 μg of total RNA of each genotype using Dynabeads mRNA Purification Kit (Invitrogen Corporation) according to the manufacturer's protocol. cDNA synthesis was performed using 2 μg of mRNA.

    Article Title: Smelly communication between haemaphysalis longicornis and infected hosts with indolic odorants: A case from severe fever with thrombocytopenia syndrome virus.
    Article Snippet: All analyses were conducted using SPSS STATISTICS version 18.0 software (SPSS Inc., Chicago, IL, USA). .. Annotation of candidate olfactory proteins and their predicted 3D structures Total foreleg RNA was isolated by trizol reagent (Invitrogen, Carlsbad, CA, USA) to construct cDNA library using the Creator SMART cDNA Library Construction Kit (Clontech, Mountain, CA, USA). .. The cDNA library was sequenced with Illumina sequencer.

    Article Title: Smelly communication between haemaphysalis longicornis and infected hosts with indolic odorants: A case from severe fever with thrombocytopenia syndrome virus
    Article Snippet: All analyses were conducted using SPSS STATISTICS version 18.0 software (SPSS Inc., Chicago, IL, USA). .. Total foreleg RNA was isolated by trizol reagent (Invitrogen, Carlsbad, CA, USA) to construct cDNA library using the Creator SMART cDNA Library Construction Kit (Clontech, Mountain, CA, USA). .. The cDNA library was sequenced with Illumina sequencer.

    cDNA Library Assay:

    Article Title: Bioinformatic analysis of ESTs collected by Sanger and pyrosequencing methods for a keystone forest tree species: oak
    Article Snippet: For each library devoted to Sanger sequencing, total RNA was extracted following the procedure described by Le Provost et al. [ ] with a final purification step using the RNAeasy kit (QIAGEN, Courtaboeuf, France). .. Depending on the quantity of each tissue, cDNA libraries were constructed using either CloneMiner cDNA Library Construction Kit (Invitrogen Corporation, Carlsbad, CA, USA) and Stratagene cDNA synthesis kit (Stratagene, La Jolla, CA, USA) when plant material was abundant, or Creator SMART cDNA Library Construction Kit (Clontech Laboratories Inc., Mountain View, CA, USA) when plant material was limiting. .. For libraries constructed with CloneMiner kit, mRNA was isolated from 200 μg of total RNA of each genotype using Dynabeads mRNA Purification Kit (Invitrogen Corporation) according to the manufacturer's protocol. cDNA synthesis was performed using 2 μg of mRNA.

    Article Title: Evolution of p-coumaroylated lignin in eudicots provides new tools for cell wall engineering.
    Article Snippet: .. Gene identification Total RNA was isolated from the fresh stems of actively growing kenaf plants using the TRIzol reagent (Invitrogen, ThermoFisher Scientific, Carlsbad, CA, USA). cDNA was synthesised using the Creator SMART cDNA library construction kit (Clontech, Mountain View, CA, USA) and was subjected to 454 sequencing using a 454 GSFLX Titanium Sequencer (Roche). ..

    Article Title: Detect Cytochrome C Oxidase- and Glutathione-S-Transferase-Mediated Detoxification in a Permethrin-Resistant Population of Lygus lineolaris
    Article Snippet: Total RNA was prepared using TriZol reagent (Invitrogen, Carlsbad, CA, USA). mRNA was purified from total RNA using the NucleoTrap mRNA purification kit (BD Bioscience Clontech, Palo Alto, CA, USA). .. The Creator Smart cDNA Library Construction Kit (BD Bioscience Clontech) was used for cDNA library construction by following the manufacturer’s instructions and modified protocols described by Zhu et al. [ ]. .. Approximately 30,000 clones were obtained and sequenced with an M13 forward primer on an ABI 3730XL sequencer. cDNA sequences were assembled using Lasergene software (DNAStar, Madison, WI, USA) and subjected to a similarity search for putative identity against GenBank protein and nucleotide databases ( http://blast.ncbi.nlm.nih.gov/Blast.cgi (accessed on 1 April 2023)). cDNA sequences were submitted to Roche NimbleGen (Roche NimbleGen, Inc., Madison, WI, USA) for the production of 72K gene expression chips in 4-plex format.

    Article Title: Smelly communication between haemaphysalis longicornis and infected hosts with indolic odorants: A case from severe fever with thrombocytopenia syndrome virus.
    Article Snippet: All analyses were conducted using SPSS STATISTICS version 18.0 software (SPSS Inc., Chicago, IL, USA). .. Annotation of candidate olfactory proteins and their predicted 3D structures Total foreleg RNA was isolated by trizol reagent (Invitrogen, Carlsbad, CA, USA) to construct cDNA library using the Creator SMART cDNA Library Construction Kit (Clontech, Mountain, CA, USA). .. The cDNA library was sequenced with Illumina sequencer.

    Article Title: Smelly communication between haemaphysalis longicornis and infected hosts with indolic odorants: A case from severe fever with thrombocytopenia syndrome virus
    Article Snippet: All analyses were conducted using SPSS STATISTICS version 18.0 software (SPSS Inc., Chicago, IL, USA). .. Total foreleg RNA was isolated by trizol reagent (Invitrogen, Carlsbad, CA, USA) to construct cDNA library using the Creator SMART cDNA Library Construction Kit (Clontech, Mountain, CA, USA). .. The cDNA library was sequenced with Illumina sequencer.

    Article Title: Generation and analysis of expressed sequence tags (ESTs) for marker development in yam ( Dioscorea alata L.)
    Article Snippet: .. The cDNA quality was tested by random cloning of the sequences using creator SMART cDNA library construction kit (Clonetech Laboratories, CA) and individual clones were sequenced using Sanger sequencing method. .. Agencourt Bioscience Corporation (MA, USA) was identified as a preferred partner to sequence the cDNA libraries from pathogen challenged leaf tissues using Roche 454 pyrosequencing technologies.

    Article Title: Spider peptides from Brachypelma smithi with slightly different amino acids at their C-terminal loops exert different insecticidal activities.
    Article Snippet: RNA was isolated with the “Total RNA Isolation System” from Promega (Madison, WI). .. Next, a full-length cDNA plasmid library (pDNR-LIB) was prepared using the Creator SMART cDNA Library Construction Kit (Clontech Laboratories, Inc., Palo Alto, CA). .. Based on the information obtained from direct peptide sequencing, oligonucleotides were synthesized using a model 391 DNA Synthesizer (Applied Biosystems, Foster City, CA): Bs2CCR5 5’- TGYTGYMGNGARTAYGARTGY-3’; Bs3CCY5 5’- TGYTGYTAYGARTAYGARTGY-3’; and BsCDKC5 5’-TGYGAYAARGAYGAYCC3’ (M, N, R, and Y represent degenerated nucleotides).

    cDNA Synthesis:

    Article Title: Bioinformatic analysis of ESTs collected by Sanger and pyrosequencing methods for a keystone forest tree species: oak
    Article Snippet: For each library devoted to Sanger sequencing, total RNA was extracted following the procedure described by Le Provost et al. [ ] with a final purification step using the RNAeasy kit (QIAGEN, Courtaboeuf, France). .. Depending on the quantity of each tissue, cDNA libraries were constructed using either CloneMiner cDNA Library Construction Kit (Invitrogen Corporation, Carlsbad, CA, USA) and Stratagene cDNA synthesis kit (Stratagene, La Jolla, CA, USA) when plant material was abundant, or Creator SMART cDNA Library Construction Kit (Clontech Laboratories Inc., Mountain View, CA, USA) when plant material was limiting. .. For libraries constructed with CloneMiner kit, mRNA was isolated from 200 μg of total RNA of each genotype using Dynabeads mRNA Purification Kit (Invitrogen Corporation) according to the manufacturer's protocol. cDNA synthesis was performed using 2 μg of mRNA.

    Isolation:

    Article Title: Evolution of p-coumaroylated lignin in eudicots provides new tools for cell wall engineering.
    Article Snippet: .. Gene identification Total RNA was isolated from the fresh stems of actively growing kenaf plants using the TRIzol reagent (Invitrogen, ThermoFisher Scientific, Carlsbad, CA, USA). cDNA was synthesised using the Creator SMART cDNA library construction kit (Clontech, Mountain View, CA, USA) and was subjected to 454 sequencing using a 454 GSFLX Titanium Sequencer (Roche). ..

    Article Title: Smelly communication between haemaphysalis longicornis and infected hosts with indolic odorants: A case from severe fever with thrombocytopenia syndrome virus.
    Article Snippet: All analyses were conducted using SPSS STATISTICS version 18.0 software (SPSS Inc., Chicago, IL, USA). .. Annotation of candidate olfactory proteins and their predicted 3D structures Total foreleg RNA was isolated by trizol reagent (Invitrogen, Carlsbad, CA, USA) to construct cDNA library using the Creator SMART cDNA Library Construction Kit (Clontech, Mountain, CA, USA). .. The cDNA library was sequenced with Illumina sequencer.

    Article Title: Smelly communication between haemaphysalis longicornis and infected hosts with indolic odorants: A case from severe fever with thrombocytopenia syndrome virus
    Article Snippet: All analyses were conducted using SPSS STATISTICS version 18.0 software (SPSS Inc., Chicago, IL, USA). .. Total foreleg RNA was isolated by trizol reagent (Invitrogen, Carlsbad, CA, USA) to construct cDNA library using the Creator SMART cDNA Library Construction Kit (Clontech, Mountain, CA, USA). .. The cDNA library was sequenced with Illumina sequencer.

    Sequencing:

    Article Title: Evolution of p-coumaroylated lignin in eudicots provides new tools for cell wall engineering.
    Article Snippet: .. Gene identification Total RNA was isolated from the fresh stems of actively growing kenaf plants using the TRIzol reagent (Invitrogen, ThermoFisher Scientific, Carlsbad, CA, USA). cDNA was synthesised using the Creator SMART cDNA library construction kit (Clontech, Mountain View, CA, USA) and was subjected to 454 sequencing using a 454 GSFLX Titanium Sequencer (Roche). ..

    Article Title: Generation and analysis of expressed sequence tags (ESTs) for marker development in yam ( Dioscorea alata L.)
    Article Snippet: .. The cDNA quality was tested by random cloning of the sequences using creator SMART cDNA library construction kit (Clonetech Laboratories, CA) and individual clones were sequenced using Sanger sequencing method. .. Agencourt Bioscience Corporation (MA, USA) was identified as a preferred partner to sequence the cDNA libraries from pathogen challenged leaf tissues using Roche 454 pyrosequencing technologies.

    other:

    Article Title: Desiccation survival in an Antarctic nematode: molecular analysis using expressed sequenced tags
    Article Snippet: A full-length cDNA library was prepared using CreatorTM SMARTTM cDNA library construction kit (Clontech, Mountain View, CA) according to the manufacturer's protocol.

    Modification:

    Article Title: Detect Cytochrome C Oxidase- and Glutathione-S-Transferase-Mediated Detoxification in a Permethrin-Resistant Population of Lygus lineolaris
    Article Snippet: Total RNA was prepared using TriZol reagent (Invitrogen, Carlsbad, CA, USA). mRNA was purified from total RNA using the NucleoTrap mRNA purification kit (BD Bioscience Clontech, Palo Alto, CA, USA). .. The Creator Smart cDNA Library Construction Kit (BD Bioscience Clontech) was used for cDNA library construction by following the manufacturer’s instructions and modified protocols described by Zhu et al. [ ]. .. Approximately 30,000 clones were obtained and sequenced with an M13 forward primer on an ABI 3730XL sequencer. cDNA sequences were assembled using Lasergene software (DNAStar, Madison, WI, USA) and subjected to a similarity search for putative identity against GenBank protein and nucleotide databases ( http://blast.ncbi.nlm.nih.gov/Blast.cgi (accessed on 1 April 2023)). cDNA sequences were submitted to Roche NimbleGen (Roche NimbleGen, Inc., Madison, WI, USA) for the production of 72K gene expression chips in 4-plex format.

    Olfactory:

    Article Title: Smelly communication between haemaphysalis longicornis and infected hosts with indolic odorants: A case from severe fever with thrombocytopenia syndrome virus.
    Article Snippet: All analyses were conducted using SPSS STATISTICS version 18.0 software (SPSS Inc., Chicago, IL, USA). .. Annotation of candidate olfactory proteins and their predicted 3D structures Total foreleg RNA was isolated by trizol reagent (Invitrogen, Carlsbad, CA, USA) to construct cDNA library using the Creator SMART cDNA Library Construction Kit (Clontech, Mountain, CA, USA). .. The cDNA library was sequenced with Illumina sequencer.

    Cloning:

    Article Title: Generation and analysis of expressed sequence tags (ESTs) for marker development in yam ( Dioscorea alata L.)
    Article Snippet: .. The cDNA quality was tested by random cloning of the sequences using creator SMART cDNA library construction kit (Clonetech Laboratories, CA) and individual clones were sequenced using Sanger sequencing method. .. Agencourt Bioscience Corporation (MA, USA) was identified as a preferred partner to sequence the cDNA libraries from pathogen challenged leaf tissues using Roche 454 pyrosequencing technologies.

    Clone Assay:

    Article Title: Generation and analysis of expressed sequence tags (ESTs) for marker development in yam ( Dioscorea alata L.)
    Article Snippet: .. The cDNA quality was tested by random cloning of the sequences using creator SMART cDNA library construction kit (Clonetech Laboratories, CA) and individual clones were sequenced using Sanger sequencing method. .. Agencourt Bioscience Corporation (MA, USA) was identified as a preferred partner to sequence the cDNA libraries from pathogen challenged leaf tissues using Roche 454 pyrosequencing technologies.

    Plasmid Preparation:

    Article Title: Spider peptides from Brachypelma smithi with slightly different amino acids at their C-terminal loops exert different insecticidal activities.
    Article Snippet: RNA was isolated with the “Total RNA Isolation System” from Promega (Madison, WI). .. Next, a full-length cDNA plasmid library (pDNR-LIB) was prepared using the Creator SMART cDNA Library Construction Kit (Clontech Laboratories, Inc., Palo Alto, CA). .. Based on the information obtained from direct peptide sequencing, oligonucleotides were synthesized using a model 391 DNA Synthesizer (Applied Biosystems, Foster City, CA): Bs2CCR5 5’- TGYTGYMGNGARTAYGARTGY-3’; Bs3CCY5 5’- TGYTGYTAYGARTAYGARTGY-3’; and BsCDKC5 5’-TGYGAYAARGAYGAYCC3’ (M, N, R, and Y represent degenerated nucleotides).



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